other:Article Title: Methotrimeprazine is a neuroprotective antiviral in JEV infection via adaptive ER stress and autophagy
Article Snippet: LC3B Abcam (ab51520, dilution 1:2000) 114. mTOR CST (2983S, dilution 1:1000) 115.
Article Title: LncTUG1 contributes to the progression of hepatocellular carcinoma via the miR-144-3p/RRAGD axis and mTOR/S6K pathway
Article Snippet: Membranes were blocked with 5% skimmed milk powder solution and incubated with antibodies including GAPDH (CST), RRAGD (CST, USA), mTOR (CST), p-mTOR (CST), p70S6K (Biogot Technology, China) and p-p70S6K (Biogot Technology), at 4°C overnight.
Blocking Assay:Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com Article ll OPEN ACCESS
Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com
Recombinant:Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com Article ll OPEN ACCESS
Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com
In Vivo:Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com Article ll OPEN ACCESS
Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com
Labeling:Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com Article ll OPEN ACCESS
Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com
Polymerase Chain Reaction:Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com Article ll OPEN ACCESS
Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com
Membrane:Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com Article ll OPEN ACCESS
Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com
Mouse Assay:Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com Article ll OPEN ACCESS
Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com
RNA sequencing:Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com Article ll OPEN ACCESS
Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com
Expressing:Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com Article ll OPEN ACCESS
Article Title: Single-cell transcriptomics identifies adipose tissue CD271 + progenitors for enhanced angiogenesis in limb ischemia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-human CD31 [WM59] BD Biosciences Cat# 560984; RRID: AB_395838 FITC anti-human CD45 [HI30] BD Biosciences Cat# 555482; RRID: AB_395874 PerCP anti-human CD34 [8G12] BD Biosciences Cat# 340430; RRID: AB_400034 APC anti-human CD271 [ME20.4–1] Miltenyi Biotec Cat# 130-113-980; RRID: AB_2661087 APC anti-human CD90 [5E10] BD Biosciences Cat# 561971; RRID: AB_398677 PerCP-Cy5.5 anti-human CD105 [266] BD Biosciences Cat# 560819; RRID: AB_2033933 PerCP-Cy5.5 anti-mouse CD31 [MEC13.3] BD Biosciences Cat# 562861; RRID: AB_2737847 BD Human Fc Block BD Biosciences Cat# 564219; RRID: AB_2728082 Akt CST Cat# 9272 p-Akt (Ser473) CST Cat# 4060 mTOR CST Cat# 2972 p-mTOR (Ser2448) CST Cat# 5536 S6K CST Cat# 9202 p-S6K (Thr421/Ser424) CST Cat# 9204 GAPDH Wako Cat# 01625523 Biological samples Human subcutaneous adipose tissue This paper N/A Human umbilical vein endothelial cells (HUVEC) Lonza Cat# CC2517A Chemicals, peptides, and recombinant proteins PKH26 Cell Linker Kit Sigma Cat# PKH26GL GSLI Isolectin B4 Vector Laboratories Cat# FL-1201-0.5 In Vivo EdU Labeling Kit Sigma Cat# BCK488-IV-IM LiberaseTM TM Research Grade Roche Cat# 5401119001 BD Pharm LyseTM Lysing Buffer BD Biosciences Cat# 555899 RNeasy kit Qiagen Cat# 74104 TaqManTM Universal PCR Master Mix Applied Biosystems Cat# 4304437 EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM Lonza Cat# CC3162 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free Corning Cat# 356231 Lipofectamine RNAiMAX Invitrogen Cat# 13778150 Human NGF-b Sigma Cat# N1408 Deposited data scRNAseq Human Adipose Hildreth et al.26 GEO: GSE155960 scRNAseq Human Adipose Vijay et al.50 GEO: GSE129363 scRNAseq Mice Adipose Rondini et al.46 GEO: GSE180589 scRNAseq Human Bone Marrow Muto et al.24 GEO: GSE184937 scRNAseq Human Umbilical Cord Blood Xiao et al.68 GEO: GSE157007 scRNAseq Human Umbilical Cord Blood Moison et al.69 GEO: GSE153370 RNAseq Human Adipose, Limb, and Skeletal Muscle Genotype-Tissue Expression (GTEx) Project gtexportal.org; dbGaP Accession phs000424.v8.p2 RNAseq Human Limb Muscle Ryan et al.45 GEO: GSE120642 (Continued on next page) Cell Reports Medicine 4, 101337, December 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNAseq Mouse Adipose Tissue and Limb Muscle Tabula Muris Consortium et al.44 https://tabula-muris.ds. czbiohub.org Experimental models: Cell Lines Human AT-CD271+/ cells This paper N/A Human ADSCs This paper N/A Mouse ADSCs This paper N/A Experimental models: Organisms/Strains Mouse: Crl:NU(NCr)-Foxn1nu Charles River Strain: 490 Mouse: B6.129S4-Ngfrtm1Jae/J The Jackson Laboratory Cat# JAX: 002213; RRID: IMSR_JAX:002213 Mouse: C57BL/6J The Jackson Laboratory Cat# JAX: 000664; RRID: IMSR_JAX:000664 Oligonucleotides SignalSilence mTOR siRNA I CST Cat# 6381 SignalSilence Control siRNA CST Cat# 6568 qPCR Primers N/A See Table S8 Software and algorithms BBrowser 3 Bioturing https://bioturing.com/single-cell FlowJo FlowJo, LCC https://www.flowjo.com The Gene Ontology Resource Gene Ontology Consortium http://geneontology.org Prism GraphPad https://www.graphpad.com
Western Blot:Article Title: The endothelial nitric oxide synthase/cyclic guanosine monophosphate/protein kinase G pathway activates primordial follicles
Article Snippet: .. Antibody Source Cat. Dilution number IF/IHC WB eNOS Novus NB300-500 1:100 nNOS CST 4231 1:100 iNOS CST 13120 1:100 PKG CST 3248 1:50 mTOR Gentex GT 649 1:50 mTOR CST 2983 1:1000 MSY2 Santa Cruz Sc-21316 1:100 FOXO3a CST 12829 1:50 FBXW7 Thermo Scientific 40-1500 1:50 1:1000 Flag Thermo Scientific F2555 1:100 GAPDH CST 2118 1:1000 IF, immunofluorescence; IHC, immunochemistry staining; WB, Western blotting. ..
Article Title: 3,4-Dimethoxychalcone, a caloric restriction mimetic, enhances TFEB-mediated autophagy and alleviates pyroptosis and necroptosis after spinal cord injury
Article Snippet: .. Antibodies/Reagents Source Concentration for WB Concentration for IF Identifier GFAP Santa Cruz / 1:200 cat# sc-33673 Beclin1 CST 1:1000 / cat# 3738 NLRP3 CST 1:1000 / cat# 15101 TMS1/ASC CST 1:1000 / cat# 67824 LC3 CST 1:1000 1:200 cat# 3868 β-actin CST 1:1000 / cat# 8457 cleaved caspase-8 CST 1:1000 / cat# 8592 acetyl-α-tubulin CST 1:1000 / cat# 5335 α-tubulin CST 1:1000 / cat# 2125 AMPK CST 1:1000 / cat# 5832 p-AMPK CST 1:1000 / cat# 2531 mTOR CST 1:1000 / cat# 2983 p-mTOR CST 1:1000 / cat# 5536 VPS34 proteintech 1:1000 / cat# 12452-1-AP cathepsin D (CTSD) proteintech 1:1000 / cat# 21327-1-AP caspase-8 proteintech 1:1000 / cat# 13423-1-AP TXNIP proteintech 1:1000 / cat# 18243-1-AP caspase-1 proteintech 1:1000 1:200 cat# 22915-1-AP histone H3 proteintech 1:1000 / cat# 17168-1-AP GAPDH proteintech 1:4000 / cat# 10494-1-AP RIPK1 Abcam 1:1000 1:200 cat# ab106393 RIPK3 Abcam 1:1000 1:200 cat# ab62344 p-MLKL Abcam 1:1000 / cat# ab196436 TRPML1/MG-2 Abcam 1:1000 / cat# ab28508 NeuN Abcam / 1:500 cat# ab177487 Iba-1 Abcam / 1:500 cat# ab283319 synaptophysin Abcam / 1:500 cat# ab32594 MAP2 Abcam / 1:500 cat# ab32454 p62/SQSTM1 Abcam 1:1000 1:200 cat# ab56416 goat anti-rabbit IgG H&L (Alexa Fluor® 488) Abcam / 1:1000 cat# ab150077 goat anti-mouse IgG H&L (Alexa Fluor® 647) Abcam / 1:1000 cat# ab150115 Goat anti-mouse IgG (H+L)-HRP Bioworld / 1:500 cat# BS12478 Goat anti-rabbit IgG (H+L)-HRP Bioworld / 1:500 cat# BS13278 IL-1β ABclonal 1:1000 / cat# A11369 TFEB ABclonal 1:1000 1:200 cat# A7311 calcineurin ABclonal 1:1000 / cat# A4346 MLKL Affinity 1:1000 / cat# DF7412 IL-18 Affinity 1:1000 / cat# DF6252 p-RIPK1 Affinity 1:1000 / cat# AF2398 p-RIPK3 Affinity 1:1000 / cat# AF7443 GSDMD Affinity 1:1000 1:200 cat# AF4013 p70S6K HuaBio 1:1000 / cat# ER31205 p-p70S6K HuaBio 1:1000 / cat# RT1456 3,4-Dimethoxychalcone Extrasynthese / / cat# ZES-1294 3-Methyladenine (3MA) Sigma‒Aldrich / / cat# M9281 Compound C Med Chem Express / / cat# 866405-64-3 MHY1485 Med Chem Express / / cat# HY-B0795 tacrolimus Med Chem Express / / cat# HY-13756 gentamicin sulfate Med Chem Express / / cat# HY-A0276 Table S1. ..
Article Title: Rg3 inhibits hypoxia-induced tumor exosomes from boosting pancreatic cancer vasculogenic mimicry through the HIF-1α/LARS1/mTOR axis
Article Snippet: .. CD9 CST 98327 WB: 1:1000 CD63 CST 52090 WB: 1:1000 HIF-1α Abcam ab51608 WB: 1:1000 IHC: 1:100 LARS1 Abcam ab31534 WB: 1:1000 IHC: 1:200 p-mTOR Abcam ab109268 WB: 1:1000 IHC: 1:100 mTOR CST 2983 1:1000 Table 3. ..
Immunofluorescence:Article Title: The endothelial nitric oxide synthase/cyclic guanosine monophosphate/protein kinase G pathway activates primordial follicles
Article Snippet: .. Antibody Source Cat. Dilution number IF/IHC WB eNOS Novus NB300-500 1:100 nNOS CST 4231 1:100 iNOS CST 13120 1:100 PKG CST 3248 1:50 mTOR Gentex GT 649 1:50 mTOR CST 2983 1:1000 MSY2 Santa Cruz Sc-21316 1:100 FOXO3a CST 12829 1:50 FBXW7 Thermo Scientific 40-1500 1:50 1:1000 Flag Thermo Scientific F2555 1:100 GAPDH CST 2118 1:1000 IF, immunofluorescence; IHC, immunochemistry staining; WB, Western blotting. ..
Immunohistochemistry:Article Title: The endothelial nitric oxide synthase/cyclic guanosine monophosphate/protein kinase G pathway activates primordial follicles
Article Snippet: .. Antibody Source Cat. Dilution number IF/IHC WB eNOS Novus NB300-500 1:100 nNOS CST 4231 1:100 iNOS CST 13120 1:100 PKG CST 3248 1:50 mTOR Gentex GT 649 1:50 mTOR CST 2983 1:1000 MSY2 Santa Cruz Sc-21316 1:100 FOXO3a CST 12829 1:50 FBXW7 Thermo Scientific 40-1500 1:50 1:1000 Flag Thermo Scientific F2555 1:100 GAPDH CST 2118 1:1000 IF, immunofluorescence; IHC, immunochemistry staining; WB, Western blotting. ..
Article Title: Rg3 inhibits hypoxia-induced tumor exosomes from boosting pancreatic cancer vasculogenic mimicry through the HIF-1α/LARS1/mTOR axis
Article Snippet: .. CD9 CST 98327 WB: 1:1000 CD63 CST 52090 WB: 1:1000 HIF-1α Abcam ab51608 WB: 1:1000 IHC: 1:100 LARS1 Abcam ab31534 WB: 1:1000 IHC: 1:200 p-mTOR Abcam ab109268 WB: 1:1000 IHC: 1:100 mTOR CST 2983 1:1000 Table 3. ..
Staining:Article Title: The endothelial nitric oxide synthase/cyclic guanosine monophosphate/protein kinase G pathway activates primordial follicles
Article Snippet: .. Antibody Source Cat. Dilution number IF/IHC WB eNOS Novus NB300-500 1:100 nNOS CST 4231 1:100 iNOS CST 13120 1:100 PKG CST 3248 1:50 mTOR Gentex GT 649 1:50 mTOR CST 2983 1:1000 MSY2 Santa Cruz Sc-21316 1:100 FOXO3a CST 12829 1:50 FBXW7 Thermo Scientific 40-1500 1:50 1:1000 Flag Thermo Scientific F2555 1:100 GAPDH CST 2118 1:1000 IF, immunofluorescence; IHC, immunochemistry staining; WB, Western blotting. ..
Concentration Assay:Article Title: 3,4-Dimethoxychalcone, a caloric restriction mimetic, enhances TFEB-mediated autophagy and alleviates pyroptosis and necroptosis after spinal cord injury
Article Snippet: .. Antibodies/Reagents Source Concentration for WB Concentration for IF Identifier GFAP Santa Cruz / 1:200 cat# sc-33673 Beclin1 CST 1:1000 / cat# 3738 NLRP3 CST 1:1000 / cat# 15101 TMS1/ASC CST 1:1000 / cat# 67824 LC3 CST 1:1000 1:200 cat# 3868 β-actin CST 1:1000 / cat# 8457 cleaved caspase-8 CST 1:1000 / cat# 8592 acetyl-α-tubulin CST 1:1000 / cat# 5335 α-tubulin CST 1:1000 / cat# 2125 AMPK CST 1:1000 / cat# 5832 p-AMPK CST 1:1000 / cat# 2531 mTOR CST 1:1000 / cat# 2983 p-mTOR CST 1:1000 / cat# 5536 VPS34 proteintech 1:1000 / cat# 12452-1-AP cathepsin D (CTSD) proteintech 1:1000 / cat# 21327-1-AP caspase-8 proteintech 1:1000 / cat# 13423-1-AP TXNIP proteintech 1:1000 / cat# 18243-1-AP caspase-1 proteintech 1:1000 1:200 cat# 22915-1-AP histone H3 proteintech 1:1000 / cat# 17168-1-AP GAPDH proteintech 1:4000 / cat# 10494-1-AP RIPK1 Abcam 1:1000 1:200 cat# ab106393 RIPK3 Abcam 1:1000 1:200 cat# ab62344 p-MLKL Abcam 1:1000 / cat# ab196436 TRPML1/MG-2 Abcam 1:1000 / cat# ab28508 NeuN Abcam / 1:500 cat# ab177487 Iba-1 Abcam / 1:500 cat# ab283319 synaptophysin Abcam / 1:500 cat# ab32594 MAP2 Abcam / 1:500 cat# ab32454 p62/SQSTM1 Abcam 1:1000 1:200 cat# ab56416 goat anti-rabbit IgG H&L (Alexa Fluor® 488) Abcam / 1:1000 cat# ab150077 goat anti-mouse IgG H&L (Alexa Fluor® 647) Abcam / 1:1000 cat# ab150115 Goat anti-mouse IgG (H+L)-HRP Bioworld / 1:500 cat# BS12478 Goat anti-rabbit IgG (H+L)-HRP Bioworld / 1:500 cat# BS13278 IL-1β ABclonal 1:1000 / cat# A11369 TFEB ABclonal 1:1000 1:200 cat# A7311 calcineurin ABclonal 1:1000 / cat# A4346 MLKL Affinity 1:1000 / cat# DF7412 IL-18 Affinity 1:1000 / cat# DF6252 p-RIPK1 Affinity 1:1000 / cat# AF2398 p-RIPK3 Affinity 1:1000 / cat# AF7443 GSDMD Affinity 1:1000 1:200 cat# AF4013 p70S6K HuaBio 1:1000 / cat# ER31205 p-p70S6K HuaBio 1:1000 / cat# RT1456 3,4-Dimethoxychalcone Extrasynthese / / cat# ZES-1294 3-Methyladenine (3MA) Sigma‒Aldrich / / cat# M9281 Compound C Med Chem Express / / cat# 866405-64-3 MHY1485 Med Chem Express / / cat# HY-B0795 tacrolimus Med Chem Express / / cat# HY-13756 gentamicin sulfate Med Chem Express / / cat# HY-A0276 Table S1. ..
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